Michael Held
Recent News
Achived News
Education
密歇根州立大学博士后
普渡大学博士后
Ph.D. Ohio University
B.S. Ohio University
Current Research
The Held Lab: Understanding the regulation and assembly of plant cell walls
Areas of Expertise
- 植物细胞壁生物化学:细胞壁生物合成和植物细胞壁自组装的转录后调控
Wall Assembly
Plant cell walls are composed of polymeric networks of cellulose, 交联聚糖, 矩阵多糖, 结构糖蛋白, 酚类化合物. 只有少数例外, 细胞壁碳水化合物聚合物是在植物高尔基体中合成的，并通过分泌途径靶向细胞壁. Upon their delivery to the wall, these polymeric networks assembly to form the cell walls. 控制壁聚合物组装的分子因素尚不清楚，因此是我实验室当前的研究重点. Specifically, 我在调查重复, block co-polymers known as extensin (EXTs) glycoproteins for their role in directing cell wall assembly, 结合生物化学和生物物理学的研究.
壁生物合成的转录后调控
Plant cell wall biosynthesis is a highly coordinated process at the gene expression level. Distinct sets of genes are activated and repressed for both primary and secondary wall biosynthesis. 调控从初级到次级细胞壁生物合成过渡的转录调控网络是相当众所周知的. However, 我实验室之前的研究表明，纤维素合酶衍生的小rna在壁生物合成的广泛转录后调控中发挥了作用. 不像转录调控网络, post-transcriptional regulation of cell wall biosynthesis is not well understood. 我的实验室目前正致力于利用小RNA下一代测序(sRNA-NGS)和生物信息学技术鉴定可以影响壁生物合成的特定小RNA.
选定的出版物
Tasleem Javaid, Matrika Bhattarai, Akshayaa Venkataraghavan, MA Held, Ahmed Faik (2024). 水稻GT43和GT47家族成员之间的特定蛋白质相互作用，形成各种假定的木聚糖合成酶复合物的核心. Plant Journal. http://doi.org/10.1111/tpj.16640
Dasmeet Kaur, MA Held, Yuan Zhang, Diana Moreira, Sílvia Coimbra, Allan M. Showalter (2023). Knockout of eight hydroxyproline-O-galactosyltransferases cause multiple vegetative and reproductive growth defects. 细胞表面10:100117. http://doi.org/10.1016/j.tcsw.2023.100117
Ryanne Oldham & MA Held (2023). Methods for Detection and Identification of Beer-Spoilage Microbes. 微生物学前缘14:1217704. http://doi.org/10.3389/fmicb.2023.1217704
谭黎，徐剑锋，马浩德，Derek T.A. 玛西娅·基利泽夫斯基《newbb电子平台》(2023). 拟南芥细胞悬浮培养表达的重复丝氨酸-羟脯氨酸糖块的阿拉伯半乳聚糖结构. Plants 12(5): 1036. http://doi.org/10.3390 / plants12051036
Diana Moreira, Dasmeet Kaur, Ana Marta Pereira, MA Held, Allan M. Showalter, Sílvia科英布拉(2023). 由羟脯氨酸- o -半乳糖转移酶引发的II型阿拉伯半乳糖在花粉-雌蕊相互作用中起重要作用. 植物学报，2014 (2):371-389. http://doi.org/10.1111/tpj.16141
周亚迪，Abhijit Sukul, John W. Mishler-Elmore, Ahmed Faik和MA Held (2022). PlantNexus: A Gene Co-expression Network Database and Visualization Tool for Barley and Sorghum. Plant & Cell Physiol. 63(4): 565-572. http://doi.org/10.1093/pcp/pcac007
Eliana Marzol, Cecilia Borassi, Mariana Carignani Sardoy, Philippe Ranocha, Ariel. A. Aptekmann, Mauro Bringas, Janice Pennington, Julio Paez-Valencia, Javier Martínez Pacheco, Diana R. Rodríguez-Garcia, Yossmayer del Carmen Rondón Guerrero, 胡安·曼努埃尔·佩拉尔塔, Margaret Fleming, John W. Mishler-Elmore, Silvina Mangano, Francisca Blanco-Herrera, Patricia A. 贝丁格，克里斯托夫·杜南，卢西亚娜·卡佩斯，亚历杭德罗·D. Nadra, MA Held, Marisa S. Otegui & José M. Estevez (2022) III类过氧化物酶PRX01, PRX44, PRX73控制拟南芥根毛生长. 中国生物医学工程学报23(10):531 - 531. http://doi.org/10.3390/ijms23105375
DTA Lamport, L Tan, MA Held, MJ Kieliszewski (2022). 根尖倾斜悖论解决了. 学术通讯，第4998条
Dasmeet Kaur, MA Held, Mountain Smith, Allan M Showalter (2021). 拟南芥合成羟基脯氨酸-半乳糖转移酶的功能表征. BMC Plant Biol. 21(1): 1-24. http://doi.org/10.1186/s12870-021-03362-2
Oyeyemi Ajayi, MA Held, [2]拟南芥中半乳多糖的糖醛酸化作用. 植物学报，2009 (1):164-181. http://doi.org/10.1111/tpj.15562
赵开元，王琪，潘夏. Gunawardena, MA Held, Ahmed Faik, and Hao Chen (2021) Desalting paper spay mass spectrometry (DPS-MS) for rapid detection of glycans and glycoconjugates. Inter. J. Mass Spec. 469: 116688. http://doi.org/10.1016/j.ijms.2021.116688
周亚迪，Abhijit Sukul, John W. Mishler-Elmore, Ahmed Faik, and MA Held (2021) PlantNexus: A Gene Co-expression Network Database and Visualization Tool for Barley and Sorghum. BioRXiv pre-print. (BIORXIV/2021/441196; submitted 4/23/21). http://doi.org/10.1101/2021.04.23.441196
Oyeyemi Ajayi, MA Held, [3] [j]拟南芥生长发育过程中β-葡萄糖醛酸转移酶基因的研究. Plants 10(6): 1172. http://doi.org/10.3390/plants10061172
Oyeyemi Ajayi, MA Held, [2] [a]拟南芥中β-葡萄糖醛酸基转移酶在2型半乳聚糖合成中的作用. 植物生物学21(1):1-19. http://doi.org/10.1186/s12870-021-03012-7
John W. Mishler-Elmore, Yadi Zhou, Abhijit Sukul, Mercedes Oblak, Li Tan and Ahmed Faik, MA Held (2021). Extensins: Self-assembly, crosslinking, and the role of peroxidases. 植物科学前沿. 12: 872. http://doi.org/10.3389/fpls.2021.664738
Yuan Zhang, MA Held, 羟基脯氨酸- o -半乳糖转移酶基因家族的CRISPR-Cas9多重基因编辑改变了拟南芥中阿拉伯半乳糖蛋白的糖基化和功能. 植物生物学21(1):1-16. http://doi.org/10.1186/s12870-020-02791-9
Daniel B. 没什么，阿比吉特·苏库尔，约翰·W. Mishler-Elmore, 和MA Held(2021)纤维素合成酶反义转录物衍生的小rna对纤维素合成酶基因的转录后调控. 植物指导5(9):e347. http://doi.org/10.1002/pld3.347
Qi Wang, Matrika Bhattarai, Pengyi Zhao, Tariq Alnsour, MA Held, Ahmed Faik, and Hao Chen (2020) Fast and Sensitive Detection of Oligosaccharides Using Desalting Paper Spray Mass Spectrometry (DPS-MS) J. Am. Soc. Mass Spectrom. Sept 10.
Daniel B. Nething, John W. 米什勒-埃尔莫尔和MA Held (2020) Post-transcriptional regulation of cellulose synthase genes by small RNAs derived from CESA antisense transcripts. BioRXiv pre-print.
Alex Jones, Yadi Zhou, MA Held和Sarah Davis (2020) 美洲龙舌兰的组织组成. Yields Greater Carbohydrates From Enzymatic Hydrolysis Than Advanced Bioenergy Crops. 植物科学进展(11):654.
张源、马浩德、艾伦·M·肖沃尔特(2020) 利用CRISPR-Cas9多路复用方法阐明三种β -葡萄糖醛酸转移酶(GLCATs)在拟南芥中作用于阿拉伯半乳糖蛋白的作用. 植物生物学20 (2):1-20.
Lamport, D. T., Tan, L.， Held, MA和Kieliszewski, M. J. (2020). 叶根形成翻开了新的一页——一个新的假说. 国际分子科学杂志, 21(3): 1145.
Nepu Saha, Kyle McGaughy, MA Held, M. Toufiq Reza (2020) Hydrothermal degradation of β-estradiol and oxytetracycline at selective reaction severities. newbb电子科学2 (9):1-9.
Ahmed Faik和MA Held (2019) Plant cell wall biochemical omics: the high-throughput biochemistry for polysaccharide biosynthesis. 植物科学286,49-56.
Lamport, D.T.; Tan, L.; Held, M.; Kieliszewski, M.J. 初代细胞壁在植物形态发生中的作用. (2018) Intl. J. Mol Sci. 19(9), 2674.
兰波特DT，谭利，霍尔德MA， & Kieliszewski MJ. (2018). Pollen tube growth and guidance: Occam's razor sharpened on a molecular arabinogalactan glycoprotein Rosetta Stone. 植物学报，2017 (2)，491- 495.
Nathan T. Reem, Gennady Pogorelko, Vincenzo Lionetti, Lauran Chambers, MA Held, Daniela Bellincampi, Olga A. Zabotina(2016)多糖阿魏酰化降低细胞壁完整性，导致植物对坏死性真菌病原体的敏感性更高. 前沿植物科学7,630.
Nan Jiang, Richard Wiemels, Aaron Soya, Rebekah Whitley, Said Bahi, MA Held and Ahmed Faik (2016) Composition, assembly, and trafficking of a wheat xylan synthase complex (XSC). 植物物理pp-01777.
蔡毅，刘鹏远，马浩德，Howard D. 德瓦尔德与陈浩(2016) Coupling Electrochemistry with Probe Electrospray Ionization Mass Spectrometry. chemphyscheme(已接受稿件).
陈玉宁，董文，谭莉，马浩德，马西娅J. Kieliszewski (2015) Arabinosylation Plays a Crucial Role in Extensin Crosslinking in vitro. 生物化学学报，32 (2)，1-13.
Sang-Jin Kim, Starla Zemelis, Suania Withers, MA Held, Curtis Wilkerson, 和Federica Brandizzi (2015) CGR2和CGR3在拟南芥的果胶甲基化和植物生长中具有关键的重叠作用. 植物学报，82(2)，208-220.
Yuning Chen, Dening Ye, MA Held, Maura Cannon, Tui Ray, Prasenjit Saha, Alexandra N. Frye, Andrew Mort, 玛西娅Kieliszewski(2015)野生型拟南芥和EXT3突变株系壁中丰富HRGPs的鉴定. 植物4(1)，85-111. doi:10.3390/plants4010085
Wen Dong, 玛西娅Kieliszewski, and MA Held (2015) Identification of the pI4.番茄中的延伸素过氧化物酶. 植物化学学报(自然科学版)，33 (2):391 - 391. doi:10.1016/j.phytochem.2014.09.015
MA Held, Nan Jiang, Debarati Basu, Ahmed Faik and Allan Showalter (2014) “Plant cell wall polysaccharides: structure and biosynthesis.见:《newbb电子》. Jean-Michel Merillon and Kishan G Ramawat, Springer Verlag, Germany. doi:10.1007/978-3-319-03751-6_73-1
Bryan W. Penning, Robert W. Sykes, Nicholas C. 克里斯托弗·巴布科克. 杜加德，马·赫尔德，约翰·F·. Klimek, Jacob Shreve, Matthew Fowler, Angela Ziebell, Mark Davis, Stephen R. Decker, Geoffrey B. Turner, Nathan S. Mosier, Nathan M. Springer, Jyothi thimapuram, Clifford F. Weil, Maureen C. McCann, Nicholas C. Carpita(2014)在玉米重组自交系群体中，酶消化木质纤维素生物质的遗传决定因素与木质素丰度的遗传决定因素是独立的. 植物生理学报，32 (4)，444 - 444.
Ahmed Faik, Nan Jiang, and MA Held (2014) “Xylan biosynthesis in plants, simply complex.” In: Carpita, NC, Buckeridge MS, and McCann MC (eds) Plants and Bioenergy, Springer, New York, pp.153-181.
Tingyue Gu, MA Held, Ahmed Faik(2013)超临界CO2和离子液体预处理生物乙醇生产中的木质纤维素生物质. 环境科学学报，34，(3):935 - 949.
Debarati Basu, Yan Liang, Xiao Liu, Klaus Himmeldirk, Ahmed Faik, 玛西娅Kieliszewski, MA Held, and Allan M. Showalter(2013)拟南芥合成半乳糖蛋白特异性羟脯氨酸- o -半乳糖转移酶的功能鉴定. 中国生物医学工程学报，2009,31 (2):444 - 444.
Federica Brandizzi和MA Held(2012)“这项研究表明，小麦和水稻中的GT61糖基转移酶家族成员能够催化在木聚糖主链上添加阿拉伯糖烷基单元.评价:[Anders N et al .. Glycosyl transferases in family 61 mediate arabinofuranosyl transfer onto xylan in grasses. 美国国家科学基金委. 2012 Jan 17; 109(3):989-93; doi: 10.1073/pnas.1115858109]. 1000细胞生物学学院，2012年3月13日.
MA Held, Evan Be, Starla Zemelis, Saunia Withers, Curtis Wilkerson, and Federica Brandizzi (2011) CGR3: A Golgi-localized protein influencing homogalacturonan methylesterification. 植物学报，48 (4):832-44.
Renata Conger, Yani Chen, Silvia Fornaciari, Carmen Faso, MA Held, Luciana Renna and Federica Brandizzi (2011) Evidence for the involvement of the Arabidopsis Sec24A, COPII涂层的组成部分, 在花粉萌发中. [J] .中国生物医学工程学报，2016,31 (2):417 - 426.
Erin Slabaugh, MA Held, and Federica Brandizzi(2011)通过内质网锚定的R2R3-MYB转录因子成员控制拟南芥根毛发育. Plant J. 67, 395-405(封面文章)
Miaoying Tian, Joe Win, Elizabeth Savory, MA Held, Federica Brandizzi, Sophien Kamoun, 和Brad Day(2011)对cubensis假operonospora 454基因组测序揭示了具有假定QXLR易位基序的效应蛋白. 植物分子与微生物的相互作用，24,543-53.
Lucia Marti, Giovanni Stefano, Kentaro Tamura, Chris Hawes, Luciana Renna, MA Held和Federica Brandizzi(2010)液泡蛋白GOLD36的一种新的错义突变导致内质网的组织缺陷和植物分泌途径中异常的蛋白质运输. Plant J 63, 901.
Carmen Faso, Yani Chen, Kentaro Tamura, MA Held, Starla Zemelis, RamuSubramanian萨拉瓦南, Eric Hummel, Leslie Kung, Elizabeth Miller, Chris Hawes和Federica Brandizzi(2009)拟南芥COPII外壳蛋白Sec24A的错义突变诱导内质网和高尔基体簇的形成. 植物细胞21,3655.
MA Held, Bryan Penning, Amanda S. Brandt, Sarah A. Kessans，杨卫东，Steven R. 斯科菲尔德和尼古拉斯. Carpita. (2008)来自纤维素合酶基因的天然反义转录物的小干扰rna调节大麦细胞壁的生物合成. 中国科学院学报(自然科学版)，2009,33 (5):534 - 534.
MA Held, Aurelia Boulaflous和Federica Brandizzi. (2008) Advances in Fluorescent Protein-Based Imaging for the Analysis of Plant Endomembranes. 植物生理学报(自然科学版)，47 (4):669 - 681.
Sally L. Hanton, Laurent Chatre, Loren Matheson, Marika Rossi, MA Held, and Federica Brandizzi. (2008)具有几乎相同蛋白质序列的植物SAR1亚型表现出不同的定位和对分泌的影响. 中国生物医学工程学报(英文版)，33 (2):393 - 398.
Athikkattuvalasu S. Karthikeyan, Deepa K. Varadarajan, Ajay Jain, MA Held, Nicholas C. 卡皮塔和卡什钱德拉. Raghothama. (2007) Phosphate starvation response and sugar signaling in Arabidopsis. Planta 225, 907-918.
Ben Wegenhart, Li Tan, MA Held, 玛西娅Kieliszewski, and Liwei Chen. (2006) Aggregate structure of hydroxyproline-rich glycoprotein (HRGP) and HRGP assisted dispersion of carbon nanotubes. Nanoscale Res Lett. 1, 154-159.
MA Held, Li Tan, Abdolreza Kamyab, Michael Hare, Elena Shpak, and Marcia J. Kieliszewski. (2004) Di-isodityrosine Is the Intermolecular Cross-link of Isodityrosine-rich Extensin Analogs Cross-linked in Vitro. 中国生物医学工程学报，2009,33 (2):444 - 444.