组织学核心设备

生物医学资源

一般的描述

The Heritage College 组织学核心设备 provides use of the following tissue preparation equipment:

• 徕卡ASP300S组织处理器
• HistoCore组织嵌入器
• 徕卡RM2235低轮廓切片机
• 徕卡RM2135高规格切片机
• 徕卡自动染色XL
• 徕卡CM1950低温恒温器

The following equipment aids with slide capture: 

• LMD徕卡6000
• Motic幻灯片扫描仪

组织学核心设备

徕卡ASP300S组织处理器

The 徕卡ASP300S is an innovative, smart processor for paraffin infiltration of tissue. The equipment can process a large number of tissue samples simultaneously, 包括福尔马林, 酒精, 二甲苯和石蜡. At the end of the process, the samples are ready for paraffin block preparation.   

HistoCore组织嵌入器

The HistoCore Arcadia is a programmable tissue​ embedding station. ​Once the infiltrations have been​ completed in the tissue processor,​ the samples are ready for block ​preparation.

徕卡RM2235显微切片机

The 徕卡RM2235 is a low-profile microtome that cuts paraffin-embedded tissues for slide preparation. The thickness of the sections can be 3 to 15 microns. 

徕卡自动染色XL 

The 徕卡自动染色XL is a hematoxylin and eosin stainer. ​

徕卡CM1950低温恒温器

This instrument is a high precision microtome, which prepares tissue sections in frozen tissue samples ​usually embedded in OCT.

LMD徕卡6000

The LMD徕卡6000 conducts laser capture microdissection and is a standard technique for sample isolation from heterogenous samples.​ It is commonly used for genomics, transcriptomics, diagnostics and proteomics applications.​

Motic幻灯片扫描仪

This slide scanner prepares digital images from slides. The images can be seen on a computer using the associated software. 

使用设施详情

费用

提供的服务

The faculty and staff of the 组织学核心设备 are highly skilled in the following services:

• 组织处理
• 石蜡块制备
• 切片
• 苏木精 & 伊红染色
• 其他组织化学染色(i.e.， PAS，三色)
• 低温切片训练

Histology Core Summary for Grant Proposals

申请实验室服务

• 下载并完成 组织学核心设施申请表.
• 将填妥的表格电邮至 Kantake纪子博士 安排服务. Please include the following in your email:
  • Brief description of the service you are requesting
  • Upcoming deadlines for your request (if applicable) 
• After receiving a scheduled time for your requested service, drop off your samples and holders in the 学术研究中心，240室, 在“进货货架”上.” A completed services form (including a billing account number) will need to be placed with the samples. Samples will not be processed without a completed form.
• You will receive an email when the services are complete.

位置

学术研究中心，240室

进入及操作指引

The facility is available to the entire newbb电子平台 research community. All users must complete a request for services form, which will be reviewed by the lab technician. 批准, the user will be contacted by the lab technician to schedule one-on-one training using their specimens. All users must complete training with the technician before being authorized to use the equipment. 设备只能通过预约使用.

用户的责任

Users must provide samples that are ready for preparation. Each user is also responsible for supplying details of any hazards (e.g., 传染性生物体, 血源性病原体, toxic chemicals) associated with the preparation in accordance to newbb电子平台实验室 & 辐射安全 规定(740-593-1666). 

Publication and Acknowledgements - Very Important

To accurately reflect the assistance this resource played in your research and to help ensure its continued funding, please include the following statement in all publications: "We acknowledge use of the newbb电子平台 Heritage College Histology Core."

Acknowledgement and Co-authorship Policies

Co-authorship on publications for staff of the Histology Core may be considered appropriate when they have provided one or more of the following:

• Significant intellectual contribution to the design of the published experiments
• Substantial practical contribution to the generation, analysis and/or interpretation of experimental data
• Indispensable technical support that contributed intellectually or scientifically to the advancement of the work

工作人员 who have made an intellectual or technical contribution not justifying authorship as defined above should be given appropriate acknowledgement in any resulting publications. This provides evidence to demonstrate our involvement in the research of multiple laboratories, which will help us retain dedicated support staff, maintain the equipment and procure new equipment in the future.

问题管理

实验室协调员
Kantake纪子博士
740.593.2352 or 740.593.2816
278A学术研究中心
 

导演
Ramiro Malgor，医学博士
740.593.2324(办公室)
202B学术研究中心